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Comparative study of normal and osteoarthritic canine synovial fluid using 500 MHz 1 H magnetic resonance spectroscopy
Author(s) -
Damyanovich A. Z.,
Staples J. R.,
Chan A. D. M.,
Marshall K. W.
Publication year - 1999
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100170211
Subject(s) - osteoarthritis , synovial fluid , chemistry , hyaluronic acid , medicine , endocrinology , alanine , nuclear magnetic resonance spectroscopy , nuclear magnetic resonance , pathology , biochemistry , amino acid , anatomy , physics , alternative medicine , organic chemistry
High resolution 1 H nuclear magnetic resonance spectroscopy has been used to investigate and compare the metabolic profiles of normal and osteoarthritic synovial fluids in a canine model of osteoarthritis. The spectra of osteoarthritic synovial fluid showed (a) increased concentiations of lactate, pyruvate, lipoproteinassociated fatty acids, and glycerol as well as the ketones hydroxybutyrate and hydroxyisobutyrate, (b) reduced levels of glucose, and (c) elevated levels of N ‐acetylglycoproteins, acetate, and acetamide compared with healthy normal canine synovial fluid. An increase was also observed in the concentrations of the amino acids alanine and isoleucine. These results suggest that (a) the intaarticular environment in canine osteoarthritis is more hypoxic and acidotic than in a normal joint, (b) lipolysis may play an increasingly important role as a source of energy in osteoarthritis, and (c) the N ‐acetylglycoprotein polymer component of synovial fluid (mostly hyaluronan) seems to be increasingly fragmented and degraded into acetate by way of an acetamide intermediate with progressive osteoarthritis. The observed changes in the biochemical profile of canine osteoarthritic synovial fluid may be useful in understanding alterations in joint metabolism consequent to arthritic diseases and helpful in identifying potential markers of osteoarthritis.