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Single local injection of recombinant fibroblast growth factor‐2 stimulates healing of segmental bone defects in rabbits
Author(s) -
Kato Takashi,
Kawaguchi Hiroshi,
Hanada Keigo,
Aoyama Ikuo,
Hiyama Yoshiyuki,
Nakamura Toshiyuki,
Kuzutani Kazuya,
Tamura Makoto,
Kurokawa Takahide,
Nakamura Kozo
Publication year - 1998
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100160605
Subject(s) - recombinant dna , bone healing , fibroblast growth factor , subcutaneous injection , microbiology and biotechnology , medicine , endocrinology , anatomy , chemistry , biology , receptor , biochemistry , gene
The effects of a single local injection of recombinant human fibroblast growth factor‐2 on the healing of segmental bone defects were evaluated in rabbits. One month after the external fixator originally designed for this experiment was installed in the tibia of the rabbit, a 3‐mm bone defect was created by an osteotomy in the middle of the tibia and 0, 50, 100, 200, or 400 μg of fibroblast growth factor‐2 in 100 μg of saline solution was injected into the defect. Injection of the growth factor increased the volume and mineral content of newly made bone at the defect in a dose‐dependent manner with significant effects at Concentrations of 100 μg or greater. These significant effects were observed at 5 weeks and later. One hundred micro‐grams of the growth factor increased the volume and mineral content of newly made bone by 95 and 36%, respectively, at 5 weeks. These results indicate that a single local injection of fibroblast growth factor‐2 stimulates the healing of segmental defects. We speculate that such an injection could be clinically useful for the healing of fractures even when the fracture gap is rather large.