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Changes in cell, matrix compartment, and fibrillar collagen volumes between growth‐plate zones
Author(s) -
Noonan Kenneth J.,
Hunziker Ernst B.,
Nessler Julie,
Buckwalter Joseph A.
Publication year - 1998
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100160416
Subject(s) - matrix (chemical analysis) , chemistry , cell , cell growth , compartment (ship) , extracellular matrix , anatomy , biology , geology , biochemistry , chromatography , oceanography
To define the contributions of changes in cell, matrix compartment, and fibrillar collagen volume to longitudinal bone growth, we measured the differences in cell, pericellular/territbrial matrix and interteritorial matrix volumes, and fibrillar collagen concentrations between the upper proliferative and lower hypertrophic zones of the proximal tibial physes of six miniature pigs. The mean numerical density of cells decreased from 110,000 cells/mm 3 in the upper proliferative zone to 59,900 cells/mm 3 in the lower hypertrophic zone. The mean cell volume increased nearly 5‐fold (from 1,174 to 5,530 um 3 ), and the total matrix volume per cell increased 46% (from 8,040 to 11,760 μm 3 /cell) between the upper proliferative and lower hypertrophic zones. Both the pericellular/territoriai matrix volume per cell and the interterritorial matrix volume per cell increased between the upper proliferative and lower hypertrophic zones; the pericellular/ territorial matrix volume per cell increased 61% (from 4,580 to 7,390μm 3 /cell), whereas the interterrritorial matrix volume per cell increased 26% (from 3,460 to 4,370μm 3 /cell). The total increase in mean cell volume of 4,356 μm 3 exceeded the total increase in mean matrix volume per cell of 3,720 μm 3 ; the total mean pericellular/territorial matrix volume per cell increased more than the total mean interterritorial matrix .volume per cell (2,810 compared with 910 μm 3 /cell). Fibrillar collagen concentration was greater in the interterritorial. matrix than in the pericellular/territoriai matrix in both zones and increased in both matrix compartments between the upper proliferative and lower hypertrophic zones. The amount of fibrillar collagen per cell also increased in both matrix compartments between the upper proliferative and lower hypertrophic zones (from;1,720 to 3,100 μm 3 /cell in the pericellular/territoriai matrix and from 1,490 to 2,230 un 3 cell in the interterritorial matrix; thus, the total amount of fibriilar collagen per cell increased from 3,210 to 5,530 μm 3 /cell). Growth rate was inversely related to the cell numerical density in the upper proliferative and lower hypertrophic zones and was directly related to interterritorial matrix volume per cell in the upper proliferative zone and to pericellular/territoriai matrix volume per cell in the lower hypertrophic zone. These results show that cell enlargement contributes more to longitudinal bone growth than does increased matrix volume, that increased pericellular/territoriai matrix volume makes a greater contribution to growth than does increased; interterritorial matrix volume, and that the total amount of fibrillar collagen per cell increases between the upper proliferative and lower hypertrophic zones. The differences between the two matrix compartments in increase in volume, fibrillar collagen concentration, and amount of fibrillar collagen per cell strongly suggest that they differ not only in matrix organization but in rate of matrix accumulation and assembly and that these differences give the two compartments different roles in skeletal growth.