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Increased content of type‐VI collagen epitopes in human ostearthritic cartilage: Quantitation by inhibition ELISA
Author(s) -
Swoboda Bernd,
Pullig Oliver,
Kirsch Thorsten,
Kladny Bernd,
Steinhäuser Bernhard,
Weseloh Gerd
Publication year - 1998
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100160116
Subject(s) - epitope , cartilage , type ii collagen , osteoarthritis , extracellular matrix , chemistry , collagen, type i, alpha 1 , matrix (chemical analysis) , type i collagen , microbiology and biotechnology , pathology , antibody , immunology , biochemistry , anatomy , medicine , biology , chromatography , alternative medicine
Type‐VI collagen is an integral part of the extracellular cartilage matrix. However, the exact amounts of type‐VI collagen in normal and osteoarthritic human cartilage still are not known. In this study, we describe an inhibition enzyme‐linked immunosorbent assay that was developed to quantitate type‐VI collagen epitopes found in guanidinium chloride extracts from normal and osteoarthritic human cartilage. In 31 cartilage samples from various localizations of healthy adult human knees, type‐VI collagen epitopes accounted for approximately 0.40% of the total collagen content. Interestingly, type‐VI collagen epitopes increased about 4‐fold in osteoarthritic cartilage. A statistically significant increase of type‐VI collagen epitopes was found during early stages of the disease, with only a superficial roughening of the cartilage surface and a loss of proteoglycans. Thus, these findings indicate that type‐VI collagen is a minor component of normal human articular cartilage and that the amount of type‐VI collagen epitopes increases significantly during early stages of osteoarthritis.