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Identification of the oim mutation by dye terminator chemistry combined with automated direct DNA sequencing
Author(s) -
Camacho Nancy P.,
Dow David,
Toledano Talya R.,
Buckmeyer Jennifer K.,
Gertner Joseph M.,
Brayton Corey F.,
Raggio Cathleen L.,
Root Leon,
Boskey Adele L.
Publication year - 1998
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100160107
Subject(s) - terminator (solar) , genotyping , microbiology and biotechnology , chemistry , dna sequencing , genotype , missense mutation , dna , genetics , mutation , gene , biology , biochemistry , ionosphere , physics , astronomy
The homozygous oimloim mouse, a model of moderate‐to‐severe human osteogenesis imperfecta, contains a G‐nucleotide deletion in the Cola‐2 gene (the murine proα2(I) collagen gene) that results in accumulation of α1(I) homotrimer collagen. Although these mice have a distinctive phenotype that includes multiple fractures and deformities, genotyping is necessary to distinguish them from their wildtype (+/+) and heterozygote ( oim/+ ) littermates. In this study, the dye primer and dye terminator chemistry methods, in combination with automated direct DNA sequencing, were compared for accuracy and ease in genotyping. A total of 82 mice from 14 litters were bred and genotyped, this resulted in 18 +/+, 35 oim /+, and 29 oim/oim mice. The dye primer and dye terminator chemistry methods worked equally well for identification of the deletion mutation and thus the genotype of all of the mice. However, the dye terminator method was found to be superior on the basis of the reduced amount of sample handling and reduced quantity of reagent required.