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Role of oxygen tension during cartilage formation by periosteum
Author(s) -
O'Driscoll Shawn W.,
Fitzsimmons James S.,
Commisso Cinzia N.
Publication year - 1997
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100150509
Subject(s) - oxygen tension , cartilage , chondrogenesis , periosteum , explant culture , chemistry , regeneration (biology) , agarose , oxygen , in vivo , anatomy , in vitro , microbiology and biotechnology , biology , biochemistry , organic chemistry
Tissue engineering makes regeneration of cartilage possible but requires optimization of culture conditions. The effects of oxygen tension on cartilage metabolism are controversial in the literature, and we could find no information detailing the optimal oxygen concentration for growing new cartilage (neochondrogenesis). Periosteal cells and tissues can be used to grow cartilage in vivo and in vitro . In this study, using a standard periosteal organ culture model, we found that cartilage formation by periosteal explants is affected by the ambient oxygen concentrations. A total of 480 periosteal explants from 30 2‐month‐old New Zealand White rabbits were cultured in agarose suspension at different oxygen concentrations (1‐90%) for 6 weeks. Chondrogenesis, which was analyzed by histomorphometry and quantitative collagen typing, was maximal at 12–15% oxygen. There were no significant differences in chondrogenesis in the range of 12–45%. There was inhibition of cartilage and type II collagen formation at very high (90%) and very low (1–5%) oxygen concentrations. However, contrary to what some have thought, chondrogenesis is maximal under aerobic conditions. If this is true for systems other than periosteal implants, it would have important implications for growing cartilage in vitro .