Premium
Stimulation of bone formation by intraosseous application of recombinant basic fibroblast growth factor in normal and ovariectomized rabbits
Author(s) -
Nakamura Kozo,
Kawaguchi Hiroshi,
Aoyama Ikuo,
Hanada Keigo,
Hiyama Yoshiyuki,
Awa Takao,
Tamura Makoto,
Kurokawa Takahide
Publication year - 1997
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100150222
Subject(s) - osteoid , basic fibroblast growth factor , fibroblast , ovariectomized rat , endocrinology , bone mineral , medicine , fibroblast growth factor 23 , apposition , femur , chemistry , mesenchymal stem cell , growth factor , fibroblast growth factor receptor 2 , fibroblast growth factor , biology , osteoporosis , microbiology and biotechnology , calcium , in vitro , surgery , biochemistry , estrogen , parathyroid hormone , receptor
Abstract The effect on intraosseous bone formation of a single local injection of recombinant human basic fibroblast growth factor into the distal femur was examined in normal and ovariectomized rabbits. In normal rabbits, basic fibroblast growth factor increased bone mineral density around the injected site in a dose‐dependent manner at 4 weeks, with significant effects at concentrations of 400 μg and greater. Doses of 400 and 1,600 μg of basic fibroblast growth factor increased bone mineral density by 8 and 9%, respectively, compared with the opposite control femur. Histological examination showed that basic fibroblast growth factor (400 μg) induced the proliferation or recruitment of undifferentiated mesenchymal cells around the existing trabeculae at 3 days after the injection. For the first 2 weeks, osteoid formation was strongly stimulated, and this was followed by mineral apposition for another 2 weeks, at which time the femurs were harvested. Consequently, basic fibroblast growth factor stimulated intraosseous bone formation at 4 weeks. We speculate that the direct action of basic fibroblast growth factor on bone formation may be to stimulate proliferation or recruitment of minimally differentiated mesenchymal cells and to initiate the cascade of events in later stages of bone formation. In ovariectomized rabbits, basic fibroblast growth factor (400 μg) also increased bone mineral density, histomorphometrical bone formation markers, and trabecular connectivity to levels similar to those in rabbits who had received sham operations.