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Expression of parathyroid hormone‐related peptide in human osteoarthritis
Author(s) -
Okano Kunihiko,
Tsukazaki Tomoo,
Ohtsuru Akira,
Osaki Makoto,
Yonekura Akihiko,
Iwasaki Katsuro,
Yamashita Shunichi
Publication year - 1997
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100150204
Subject(s) - parathyroid hormone , cartilage , osteoarthritis , medicine , in situ hybridization , hormone , endocrinology , peptide , parathyroid hormone related protein , immunohistochemistry , pathology , gene expression , biology , anatomy , gene , calcium , biochemistry , alternative medicine
To evaluate the involvement of the expression of parathyroid hormone‐related peptide gene in human articular cartilage pathology, we performed immunohistochemical staining and in situ hybridization on specimens of femoral head cartilage obtained from 15 patients with osteoarthritis, 11 with rheumatoid arthritis, and 12 control subjects. Parathyroid hormone‐related peptide‐positive chondrocytes were observed predominantly in degenerated lesions of osteoarthritic tissue and were less evident in rheumatoid arthritic samples, while the normal cartilage expressed little parathyroid hormone‐related peptide. In addition, the level of parathyroid hormone‐related peptide expression was clearly dependent on the degree of cartilage degeneration; cartilage tissues with moderate degenerative changes contained more positive chondrocytes compared with mildly or severely degenerated cartilage. In situ hybridization confirmed the localization of parathyroid hormone‐related peptide protein and demonstrated intense expression of mRNA of the peptide in osteoarthritic samples. This is the first demonstration of parathyroid hormone‐related peptide expression in chondrocytes from pathologic articular cartilage of humans. Our results, suggest that parathyroid hormone‐related peptide may be involved in the pathophysiology of osteoarthritis.