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Chondrocyte survival in cryopreserved osteochondral articular cartilage
Author(s) -
Ohlendorf Christian,
Tomford William W.,
Mankin Henry J.
Publication year - 1996
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100140311
Subject(s) - chondrocyte , cartilage , dimethyl sulfoxide , cryopreservation , articular cartilage , staining , chemistry , extracellular matrix , anatomy , pathology , andrology , biomedical engineering , microbiology and biotechnology , medicine , biology , osteoarthritis , embryo , biochemistry , alternative medicine , organic chemistry
Fluorescent staining techniques were used in combination with confocal and conventional fluorescent microscopy to determine the location of viable chondrocytes in frozen and thawed osteochondral articular cartilage. The results showed that cell survival was confined to the superficial layer of the cartilage matrix. The addition of a cryopreservative agent (dimethyl sulfoxide) increased chondrocyte survival but only in the same layer. There was no evidence of cell survival in the middle or deep layers of the cartilage with or without the use of dimethyl sulfoxide. Under the conditions employed in this study, chondrocyte survival in a cryopreserved osteochondral allograft appears to be limited to the superficial layer of the articula cartilage.