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Human monocyte response to particulate biomaterials generated in vivo and in vitro
Author(s) -
Shanbhag Arun S.,
Jacobs Joshua J.,
Black Jonathan,
Galante Jorge O.,
Glant Tibor T.
Publication year - 1995
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100130520
Subject(s) - polyethylene , zymosan , titanium , magnesium , membrane , monocyte , materials science , in vitro , interleukin , biophysics , chemistry , in vivo , immunology , biochemistry , cytokine , medicine , biology , composite material , metallurgy , microbiology and biotechnology
We studied the ability of four clinically relevant particle species to stimulate human peripheral blood monocytes to release bone‐resorbing agents, including interleukin‐1 (both interleukin‐1α and interleukin‐1β), interleukin‐6, and prostaglandin E 2 . The species studied were titanium‐6%aluminum‐4%vanadium (TiAlV), commercially pure titanium, fabricated ultrahigh molecular weight polyethylene, and polyethylene retrieved from interfacial membranes of failed uncemented total hip arthroplasties. For all species, the mean size was less than 1 μm. Human peripheral blood monocytes were challenged with these particles in a uniform manner on the basis of surface area. Phorbol 12‐myristate acetate, zymosan, and nonphagocytosable titanium particles served as controls. Stimulation of human monocytes is a function of the composition and concentration of particles. In this study. TiAlV particles appeared to be the most competent to elicit the synthesis and release of inflammatory mediators. Particles of commercially pure titanium and of fabricated ultrahigh molecular weight polyethylene also could induce the release of various cellular mediators, albeit at a lower level, whereas the particles of polyethylene retrieved from interfacial membranes were less stimulatory in these short‐term in vitro experiments.