Premium
Osteosarcoma oncogene expression detected by in situ hybridization
Author(s) -
Wang Humin,
Rodgers William H.,
Chmell Michael J.,
Svitek Chris,
Schwartz Herbert S.
Publication year - 1995
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100130506
Subject(s) - osteosarcoma , in situ hybridization , pathology , biology , sarcoma , oncogene , immunohistochemistry , cancer research , microbiology and biotechnology , gene expression , cell , gene , medicine , cell cycle , genetics , biochemistry
Abstract Fifteen archival human osteosarcoma specimens were examined by in situ hybridization for the expression of human and mouse transforming growth factor‐β (isoforms 1, 2, and 3), c‐fos, and metalloproteinase (stromelysin‐3 and matrilysin). Osteosarcoma subtypes were confirmed by review of patients' radiographs, histopathology, and age at diagnosis. The outcome and method of treatment were documented. The subtypes of osteosarcoma consisted of nine conventional osteosarcomas and two each of fibroblastic, telangiectatic, and post‐radiation osteosarcomas. Each specimen was histologically examined under light microscopy, and then adjacent paraffin sections were assayed with sense and anti‐sense RNA probes by in situ hybridization. The probes localized to the neoplastic cells, confirming the methodology of the technique. Human transforming growth factor‐β1 had the most uniform binding affinity to the osteosarcomas examined and was more specific in binding than mouse transforming growth factor‐β1. Specific mRNA encoding for the transforming growth factor‐βs, c‐fos, and metalloproteinases are detectable in patterns within osteosarcoma cells, and collectively, their expression parallels the different histopathologic subtypes. The less differentiated subtypes (telangiectatic and post‐radiation osteosarcomas) expressed the fewest molecular markers. Osteosarcoma is a heterogeneous tumor. Differential expression of matrilysin in osteosarcoma is the first reported detection of metalloproteinase activity in human skeletal sarcoma.