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Primary culture of microvascular endothelial cells from canine meniscus
Author(s) -
Miller Raymond R.,
Rydell Pamela A.
Publication year - 1993
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100110618
Subject(s) - matrigel , endothelial stem cell , immunostaining , pathology , von willebrand factor , laminin , microbiology and biotechnology , endothelium , chemistry , anatomy , biology , cell culture , extracellular matrix , medicine , immunohistochemistry , immunology , in vitro , platelet , endocrinology , biochemistry , genetics
Microvascular endothelial cells were isolated and cloned from canine knee menisci. Intitially, the endothelial cell colonies in culture exhibited a cobblestone morphology. With passage and time, these cultures adopted a fibroblastic or fusiform morphology. The endothelial nature of the cells was demonstrated by positive immunostaining with antibodies to von Willebrand factor, laminin, and type‐IV collagen and by capillary‐like tube formation on Matrigel. These endothelial cells could be passaged as many as 10 times with retention of the endothelial characteristics. This population of meniscal microvascular endothelial cells will facilitate studies on the role of blood vessels in meniscal repair.