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In vivo effects of naproxen on composition, proteoglycan metabolism, and matrix metalloproteinase activities in canine articular cartilage
Author(s) -
Ratcliffee Anthony,
Azzo Walid,
SaedNejad Fatemeh,
Lane Nancy,
Rosenwasser Melvin P.,
Mow Van C.
Publication year - 1993
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100110203
Subject(s) - proteoglycan , cartilage , aggrecan , naproxen , in vivo , matrix metalloproteinase , collagenase , gelatinase , tissue inhibitor of metalloproteinase , matrix metalloproteinase 3 , chemistry , osteoarthritis , medicine , endocrinology , pharmacology , biochemistry , articular cartilage , pathology , anatomy , enzyme , biology , alternative medicine , microbiology and biotechnology
Abstract Naproxen is a nonsteroidal anti‐inflammatory drug commonly used in the clinical treatment of joint disease. In this study, its effect in vivo on the biochemical composition, metabolic activities, and metalloproteinase activities of normal canine articular cartilage was analyzed. The articular cartilage from the knee joints of dogs who had been given naproxen for 4 weeks to maintain a serum level of 40–50 μg/ml was examined. Control animals were given a placebo. Treatment with naproxen was not found to change the composition (water, collagen, and proteoglycan) of the articular cartilage. The culture studies of cartilage explants indicated that proteoglycan synthesis rates were unaffected by the treatment with naproxen but that proteoglycan release from the tissue was suppressed. Analysis of the cartilage for matrix metalloproteinase activities showed reduced activity of neutral matrix metalloproteinase by 80%, of collagenase by 40%, and of gelatinase by 87%, with no change in activity of acid metalloproteinase or of tissue inhibitor for metalloproteinase. These findings indicate that in vivo treatment with naproxen has the capacity to modulate catabolic activities in articular cartilage.