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Colony‐forming efficiency response of bone marrow stromal cells to acute blood loss
Author(s) -
Lippiello L.,
Chavda D.,
Connolly J.
Publication year - 1992
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100100117
Subject(s) - stromal cell , bone marrow , alkaline phosphatase , immunology , erythropoietin , endocrinology , medicine , biology , andrology , enzyme , biochemistry
Colony‐forming efficiency (CFE) was used to monitor the proliferative response of alkaline phosphatase‐positive rabbit bone marrow stromal cells to acute blood loss. The CFE of animals subjected to a 1% blood loss was 0.97 compared with 0.06 (p < 0.01) in nonbled animals. Sera obtained from animals 10 days after an initial blood loss stimulated the CFE of marrow cultures from nonbled donors to the same degree as osteogenin. Erythropoietin and control sera (from nonbled animals) had no effect. Hence, acute blood loss and sera from bled animals stimulate proliferation of alkaline phosphatase‐positive marrow stromal cell colonies. The agent(s) responsible is unknown but it is present in serum in response to blood loss. Confirmation of a specific effect on osteoprogenitor cells may warrant the designation “osteopoietin”.