Premium
The effect of oxygen tension on proteoglycan synthesis and aggregation in mammalian growth plate chondrocytes
Author(s) -
Clark Charles C.,
Tolin Brad S.,
Brighton Carl T.
Publication year - 1991
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100090403
Subject(s) - proteoglycan , oxygen tension , glycosaminoglycan , in vitro , chemistry , oxygen , biophysics , microbiology and biotechnology , tension (geology) , in situ , biochemistry , anatomy , biology , extracellular matrix , materials science , organic chemistry , ultimate tensile strength , metallurgy
Growth plate chondrocytes isolated from the proliferative and hypertrophic zones of bovine costochondral junctions were grown in vitro in the presence of various oxygen tensions ranging from 3 to 60%. Using [ 35 S] sulfate as an index of glycosaminoglycan synthesis, incorporation was found to be maximal at 21% O 2 . In contrast, proteoglycan aggregation under the same conditions was found to be maximal at 3% O 2 . There were no consistent differences in response between cells from the different morphologic zones even though they are exposed to different oxygen tension in situ. These results show that proteoglycan synthesis and aggregation in growth plate chondrocytes in vitro are differentially affected by the ambient oxygen environment.