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Synthesis and characterization of magnetically responsive albumin microspheres containing cis ‐hydroxyproline for scar inhibition
Author(s) -
Iannotti J. P.,
Baradet T. C.,
Tobin M.,
Alavi A.,
Staum M.
Publication year - 1991
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100090316
Subject(s) - hydroxyproline , microsphere , albumin , chemistry , in vitro , proline , in vivo , biophysics , biochemistry , amino acid , biology , chemical engineering , microbiology and biotechnology , engineering
Magnetically responsive albumin microspheres containing the proline analog, cis ‐hydroxyproline, were synthesized and their in‐vitro physical properties were characterized. These microspheres have an average size of 1.1 ± 0.3 pm with 94% of the microspheres <2.0 pm. They are uniformly spherical and contain 67% albumin, 22% magnetite, and 8% cis ‐hydroxyproline. The cis ‐hydroxyproline is releasable during a 72‐h period and demonstrates a slow, releasable pool that constitutes approximately 50% of the incorporated drug. cis ‐Hydroxyproline is neither chemically nor biologically altered during its incorporation into the microsphere. cis ‐Hydroxyproline release from microspheres results in the in vitro inhibition of collagen secretion, which is indistinguishable from unincorporated cis ‐hydroxyproline. In a rat‐tail animal model these microspheres were selectively targeted using an external magnetic field applied to a 1.5–2.0‐cm target site. Fifty to 80% of the infused microspheres were localized to this site, whereas without a magnetic field only 15–20% of the microspheres are localized to this site. cis ‐Hydroxyproline microspheres resulted in a 16% decrease in collagen content in a scar model when compared with untreated animals (p < 0.05). With further refinement of this method of drug delivery, clinically useful inhibition of scar formation may result.