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The effects of hydrostatic pressure on matrix synthesis in articular cartilage
Author(s) -
Hall A. C.,
Urban J. P. G.,
Gehl K. A.
Publication year - 1991
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100090102
Subject(s) - cartilage , hydrostatic pressure , chemistry , matrix (chemical analysis) , articular cartilage , biophysics , stimulation , hydrostatic equilibrium , incubation , biomedical engineering , anatomy , chromatography , medicine , osteoarthritis , biochemistry , biology , pathology , thermodynamics , physics , alternative medicine , quantum mechanics
The direct effects of hydrostatic pressure on matrix synthesis in articular cartilage can be studied independently of the other factors that change during loading. We have found that the influence of hydrostatic pressure on incorporation rates of 35 SO 4 and [ 3 H]proline into adult bovine articular cartilage slices in vitro depends on the pressure level and on the time at pressure. Pressures in the “physiological” range (5–15 MPa) applied for 20s or for 5 min could stimulate tracer incorporation (30–130%) during the following 2 h, but higher pressures (20–50 MPa) had no effect on incorporation rates. The degree of stimulation in cartilage obtained from different animals was found to vary; in some animals none was seen. Stimulation also varied with position along the joint. Physiological pressures (5–10 MPa) applied continuously for the 2‐h incubation period also stimulated incorporation rates, but pressures >20 MPa always produced a decrease that was related to the applied pressure and that was reversible. These results suggests that the hydrostatic pressure that occurs during loading is a signal that can stimulate matrix synthesis rates in articular cartilage.