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Attachment of epiphyseal cartilage cells and 17/28 rat osteosarcoma osteoblasts using mussel adhesive protein
Author(s) -
Fulkerson John P.,
Norton Louis A.,
Gronowicz Gloria,
Picciano Paul,
Massicotte J. Mathieu,
Nissen Carl W.
Publication year - 1990
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100080603
Subject(s) - adhesive , cartilage , mussel , osteosarcoma , osteoblast , cell culture , microbiology and biotechnology , chemistry , cell , anatomy , biology , materials science , biochemistry , in vitro , composite material , cancer research , genetics , fishery , layer (electronics)
These experiments show that mussel adhesive protein (MAP) enhances the attachment of osteoblasts and epiphyseal cartilage cells to plastic culture dishes and Vitallium. When MAP was applied to culture plate surfaces, there were two‐ to fivefold increases in the numbers of cells attaching compared to control surfaces (no MAP). Results were confirmed using two different cell attachment assay techniques. Osteoblast replication and culture on MAP is possible, suggesting that MAP is not toxic to cells. MAP also holds applied cells to surfaces as initially attached.