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In vitro and in vivo effects of metal chelators on cartilage metabolism
Author(s) -
Ehrlich Michael G.,
Stefanich Ray,
Ishizue Kenneth K.,
Armstrong Ann L.,
Mankin Henry J.
Publication year - 1990
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100080109
Subject(s) - in vivo , in vitro , metabolism , cartilage , chemistry , metal , biochemistry , microbiology and biotechnology , biology , anatomy , organic chemistry
The effect of metallic chelating agents (EDTA, EGTA) on cartilage metabolism was studied both in vitro, on calf cartilage, and in vivo, in rabbits. The question asked was whether it was possible to affect neutral protease activity, and not also inhibit beneficial synthetic systems. In vitro, EDTA suppressed anabolic processes, while EGTA had no effect. However, EDTA in vivo did not suppress glycosaminoglycan or RNA production, but paradoxically stimulated them. At the same time, EDTA inhibited neutral protease activity in normal animals.