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Cytosolic ionized calcium concentration in isolated chondrocytes from each zone of the growth plate
Author(s) -
Iannotti J. P.,
Brighton C. T.
Publication year - 1989
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100070408
Subject(s) - calcium , cytosol , chemistry , intracellular , chondrocyte , cartilage , calcium in biology , biophysics , calcium metabolism , mineralization (soil science) , extracellular , hyaline cartilage , cell growth , endocrinology , biochemistry , anatomy , biology , in vitro , osteoarthritis , pathology , medicine , articular cartilage , alternative medicine , organic chemistry , enzyme , nitrogen
In isolated chondrocytes from the growth plate, there is an increase in both the cytosolic ionized calcium concentration and in total cell calcium content as the cells approach the mineralization front. The reserve zone chondrocytes have a cytosolic ionized calcium concentration, [Ca 2+ ] i , of 124 ± 23 n M and a total cell calcium content, Ca T , of 12.8 ± 6.8 nmol/mg cell protein. Proliferative zone chondrocytes have a [Ca 2+ ] i of 172 ± 29 n M and a Ca T of 16.1 ± 11.8 nmol/mg cell protein. Hypertrophic zone chondrocytes have a [Ca 2+ ] i of 273 ± 49 n M and a Ca T of 35.8 ± 16 nmol/mg protein. Chondrocytes isolated from the entire growth plate have a [Ca 2+ ] i of 132 ± 29 n M and a Ca T of 13.2 ± 2.5 nmol/mg cell protein. Chondrocytes isolated from noncalcifying hyaline cartilage have a [Ca 2+ ] i of 85 ± 10 n M and a Ca T of 11.5 ± 1.4 nmol/mg cell protein. There appears to be a correlation between intracellular calcium accumulation, an increase in the intracellular ionized calcium concentration, and the process of cartilage matrix mineralization.