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Growth hormone stimulates insulin‐like growth factor I actions on adult articular chondrocytes
Author(s) -
Smith R. Lane,
Palathumpat Marykutty V.,
Ku Christine W.,
Hintz Raymond L.
Publication year - 1989
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100070207
Subject(s) - chondrocyte , proteoglycan , endocrinology , growth factor , medicine , extracellular matrix , insulin like growth factor , chemistry , somatomedin , keratan sulfate , dna synthesis , microbiology and biotechnology , growth hormone , hormone , biology , biochemistry , dna , in vitro , receptor
We report effects of adding insulin‐like growth factor I (IGF‐I) and methionyl human growth hormone (GH), alone or in combination, to adult bovine articular chondrocytes plated at high density. Purified human and synthetic IGF‐I stimulated chondrocyte DNA and proteoglycan synthesis. GH had no effect on either process. However, GH added in combination with IGF‐I increased proteoglycan, cell‐associated proteoglycan, and keratan sulfate synthesis over levels observed with IGF‐I alone. IGF‐I and GH did not alter the hydrodynamic size of proteoglycans or synthesis of collagen. Our results show that GH and IGF‐I act together to stimulate adult chondrocyte extracellular matrix synthesis.