Premium
The effects of demineralized bone matrix and direct current on an “in vivo” culture of bone marrow cells
Author(s) -
Friedenberg Zachary B.,
Brighton Carl T.,
Michelson James D.,
Bednar John,
Schmidt Richard,
Brockmeyer Thomas
Publication year - 1989
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100070104
Subject(s) - demineralized bone matrix , cartilage , bone marrow , in vivo , chemistry , anatomy , matrix (chemical analysis) , bone matrix , mineralization (soil science) , biomedical engineering , pathology , materials science , medicine , biology , dbm , amplifier , microbiology and biotechnology , optoelectronics , organic chemistry , cmos , chromatography , nitrogen
Bone marrow cells (BMCs) from rabbit femora and tibiae were grown in diffusion chambers implanted in rabbit muscle. At 42 days 80% of the BMC chambers exhibited cartilage formation within them. Demineralized bone matrix added to the marrow cell suspension in the chamber accelerated the appearance and increased the number of chambers with cartilage. Mineralization of the cartilage also occurred earlier in the chambers with bone matrix. In a second experiment, a 5‐μA direct current cathode in the bone marrow chamber increased the number of chambers containing cartilage from 50 to 80% at day 25. Mineralization also occurred earlier in the chambers with direct current.