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Effect of direct current on cultured growth cartilage cells in vitro
Author(s) -
Okihana Hiroyuki,
Shimomura Yutaka
Publication year - 1988
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100060511
Subject(s) - stimulation , dna synthesis , in vitro , proteoglycan , cartilage , chemistry , thymidine , staining , cell growth , dna , microbiology and biotechnology , sodium , toluidine , biophysics , biochemistry , endocrinology , biology , anatomy , medicine , pathology , organic chemistry
Cultured growth cartilage cells of young rabbits were stimulated by three levels(10, l and 0.l μA) of constant direct current (DC). The effect of DC was examined by measuring the activities of proteoglycan synthesis (uptake of [ 35 S]sodium sulfate and toluidine blue staining) and DNA synthesis (uptake of [ 3 H]thymidine). DC increased syntheses of both proteoglycans and DNA when l μA calculated curren densiy of l μA/cm 2 was applied for 3 days. A longer stimulation resulted in a larger difference between stimulated and unstimulted cells. Ten‐microampere stimulation devitalized the cells, while 0.l μA had no effect on cell activities.