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Response of cultured bone cells to a capacitively coupled electric field: Inhibition of cAMP response to parathyroid hormone
Author(s) -
Brighton Carl T.,
McCluskey William P.
Publication year - 1988
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100060414
Subject(s) - parathyroid hormone , endocrinology , medicine , stimulation , bone resorption , desensitization (medicine) , chemistry , calcium , receptor
Fetal rat calvarial bone cells grown in monolayers were exposed to a capacitively coupled electrical field of 2.62 mV/cm for 2.5–30 min. There was a 59% increase in cAMP concentration after electrical stimulation, compared with a 3615% increase after parathyroid hormone (PTH) adminstration. PTH administration after electrical stimulation resulted in less cAMP synthesis than following either electrical stimulation alone or PTH administration alone. When PTH administration was delayed for 30 min following cessation of the electrical stimulation, there was an inhibition in the cAMP response. This suggests that one possible mechanism of electrically induced osteogenesis is the desensitization of the bone cell to PTH. Since the effect of PTH is to increase bone resorption, desensitizing or blocking the effect of PTH at the bone cell level would result in a net increase in bone formation.