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The synovial production of collagenase and chondrocyte activating factors in response to cobalt
Author(s) -
Ferguson Gary M.,
Watanabe Shoji,
Georgescu Helga I.,
Evans Christopher H.
Publication year - 1988
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100060408
Subject(s) - gelatinase , collagenase , chondrocyte , stimulation , chemistry , prostaglandin e2 , prostaglandin , medicine , endocrinology , andrology , biochemistry , enzyme , in vitro
Addition of CoCl 2 solutions to the culture media of confluent monolayers of lapine or human synoviocytes stimulated their production of the neutral proteinases collagenase, gelatinase, and caseinase. With lapine cells, maximum stimulation occurred at 10 −7 M CoCl 2 , while human cells required 10 −4 –10 −5 M CoCl 2 to achieve a maximum stimulation. Production of prostaglandin E 2 by lapine cells was enhanced some 30–40% by concentrations of CoCl 2 that maximally stimulated synthesis of the neutral proteinases, whereas all concentrations of CoCl 2 slightly depressed the productio of prostaglandin E 2 by human celis. Lapine synovial cells that had been stimulated by CoCl 2 also produced a substance, or substances, that provoked the synthesis of collagenase, gelatinase, caseinase, and prostaglandin E 2 by monolayers of articular chondrocytes. Chondrocytes themselves, however, resisted activation by CoCl 2 . These findings may be relevant to the aseptic loosening of joint prostheses.