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Degradative pathways in cultured synovial fibroblasts: Selective effects of pulsed electromagnetic fields
Author(s) -
Murray J. Clifford,
Lacy Margaret,
Jackson Sylvia Fitton
Publication year - 1988
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100060104
Subject(s) - peripheral blood mononuclear cell , collagenase , connective tissue , cytokine , prostaglandin e2 , microbiology and biotechnology , chemistry , population , cell , fibroblast , pathology , immunology , biology , endocrinology , medicine , enzyme , biochemistry , in vitro , environmental health
A cell culture model for studying the cytokine‐mediated degradation of connective tissue was exposed to clinically applied, low‐frequency pulsed electromagnetic fields (PEMF), and levels of collagenolytic activity, two lysosomal hydrolases, and prostaglandin E 2 were measured. PEMFs reduced the release of two lysosomal enzymes by cultured rabbit synovial fibroblasts but did not affect their response to mononuclear‐cell–conditioned medium. PEMF did not alter levels of cytokine activity produced by a mixed mononuclear cell population, nor did they affect the cytokine‐mediated release of collagenase or prostaglandin E 2 by synovial fibroblasts. The relevance of these findings to the clinical application of PEMF to soft‐and hard‐tissue injuries is discussed.