Premium
Streaming potentials: A sensitive index of enzymatic degradation in articular cartilage
Author(s) -
Frank Eliot H.,
Grodzinsky Alan J.,
Koob Thomas J.,
Eyre David R.
Publication year - 1987
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100050405
Subject(s) - degradation (telecommunications) , articular cartilage , index (typography) , chemistry , enzyme , cartilage , biophysics , chromatography , biomedical engineering , computer science , biochemistry , biology , medicine , anatomy , pathology , osteoarthritis , world wide web , telecommunications , alternative medicine
Abstract Under physiological conditions, the extracellular matrix of articular cartilage contains a high fixed‐charge density, associated with its ionized proteoglycan (PG) molecules. Compression of the highly charged cartilage matrix within the physologic range leads to the production of electrical streaming potentials. We observed significant changes in the potential response due to chemical modifications of the matrix, such as extraction of PG and glycosaminoglycan (GAG) moieties using chondroitinase‐ABC and trypsin. The streaming potential was a sensitive index of the degradative loss of these matrix constituents and of the kinetics of the enzymatic degradative process.