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Structure of newly synthesised ( 35 S)‐proteoglycans and ( 35 s)‐proteoglycan turnover products of cartilage explant cultures from dogs with experimental osteoarthritis
Author(s) -
Carney Stephen L.,
Billingham Michael E. J.,
Muir Helen,
Sandy John D.
Publication year - 1985
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100030203
Subject(s) - cartilage , explant culture , proteoglycan , hyaluronic acid , osteoarthritis , glycosaminoglycan , chemistry , chondroitin sulfate , anatomy , biochemistry , pathology , medicine , in vitro , alternative medicine
The structure of newly synthesised proteoglycans from explant cultures of cartilage from joints subjected to transection of the anterior cruciate ligament (osteoarthritic) and from normal (non‐ or sham‐operated) joints was examined. The structure of the products of proteoglycan turnover was also examined using explants of normal and osteoarthritic cartilage maintained in culture for a 48 h chase period. The findings were as follows: (a) Newly synthesised ( 35 S)‐proteoglycans extracted from cartilage explants from osteoarthitic joints whether examined 3 weeks, 3 months, or 6 months after surgery were larger than those from corresponding normal cartilage. This can be explained by the synthesis in osteoarthritic cartilage of abnormally long chondroitin sulphate chains on newly synthesised proteoglycans. (b) The extracts also contained a newly formed small proteoglycan species that was unable to interact with hyaluronic acid. The porportion of thei species was higher in osteoarthritic cartilage compared with normal, examined 3 weeks after surgery, but was generally absent from cartilage obtained 3 and 6 months after surgery. (c) Compared with controls, a smaller proportion of the ( 35 S)‐ proteoglycans released into the maintenance medium of explant cultures of osteoarthritic cartilage during a 48 h chase period was able to interact with hyaluronic acid. However, although furnished with longer ( 35 S)‐glycosaminoglycan chains, these proteoglycans were smaller than those from control explants.