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Cell culture of rabbit meniscal fibrochondrocytes: Proliferative and synthetic response to growth factors and ascorbate
Author(s) -
Webber Richard J.,
Harris Mary G.,
Hough Aubrey J.
Publication year - 1985
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100030104
Subject(s) - microbiology and biotechnology , fibroblast growth factor , cell culture , cell growth , chemistry , sulfation , population , glycosaminoglycan , fibroblast , in vitro , doubling time , ascorbic acid , biochemistry , matrix (chemical analysis) , biology , genetics , receptor , medicine , food science , environmental health , chromatography
This study was undertaken to determine whether the cells of the fibrocartilaginous meniscal substance are capable of proliferation and matrix synthesis. Cells were isolated from the fibrocartilaginous menisci of young New Zealand white rabbits, and grown in two alternative culture regimens differing only in the basal nutrient medium used to initiate primary monolayer growth. Under each culture regimen, the cells attached and proliferated both initially and after passage into secondary (2°) culture. Differences were noted in cell morphology and time to reach confluence in primary (1°) culture. Upon passage into 2° culture, the fibrochondrocytes assumed two distinct morphological changes were accompanied by differences in the population doubling time and incorporation of 35 SO 4 into sulfated proteoglycans. The proliferation of both fibrochondrocyte subtypes was stimulated by the addition of either pituitary fibroblast growth factor (FGF) or human platelet lysate in a dosedependent manner. Both FGF (10 ng/ml) and ascorbate (40 μg/ml) decreased 35‐sulfate incorporation, whereas only ascorbate was found to alter the amount of sulfated glycosaminogly can in the pericellular coat. We conclude that the fibrochondrocytes of the meniscal substance are capable of replication and synthesis of matrix macromolecules if given the proper stimuli. Additionally, there may be two subpopulations of fibrochondrocytes that can be distinguished by their in vitro behavior.