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Microinjury to the synovial membrane may cause disaggregation of proteoglycans in rabbit knee joint articular cartilage
Author(s) -
Frost Linda,
Ghosh Peter
Publication year - 1984
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100020302
Subject(s) - cartilage , hyaluronic acid , chemistry , articular cartilage , saline , synovial membrane , glycosaminoglycan , proteoglycan , uronic acid , anatomy , chromatography , osteoarthritis , biochemistry , medicine , endocrinology , pathology , biology , polysaccharide , arthritis , alternative medicine
Proteoglycans (PGs) isolated from articular cartilage (AC) of mature rabbits subjected to two or more consecutive intraarticular (IA) injections of sterile saline 24 h apart showed an aggregation defect in the presence of excess hyaluronic acid (HA). Although the PG contents of experimental and control cartilages were indistinguishable, a higher proportion of PGs were extractable from the 3 × IA tissues, as assessed by uronic acid analysis. Proteoglycans from experimental and control cartilages when examined by Sepharose CL‐2B chromatography showed two subunit populations, the smaller (K AV = 0.70) containing more ketatan sulphate than the larger (K AV = 0.31). Cultures of AC from IA joints released more 35 SO 4 ‐labelled PGs into the media over 72 h than control tissues and consisted mainly of PG degradation products although 20% could aggregate in the presence of HA. Examination of PG aggregation 2 weeks after 2 × IA or 3 × IA injections showed that the defect initiated was still present; however, cartilage of immature rabbits was not affected by the 2 × IA procedure.