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Comparison of growth‐induced resorption and denervation‐induced resorption on the release of [ 3 H]tetracycline, 45 calcium, and [ 3 H]collagen from whole bones of growing rats
Author(s) -
Klein L.,
Heiple K. G.,
Stromberg B. V.
Publication year - 1983
Publication title -
journal of orthopaedic research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.041
H-Index - 155
eISSN - 1554-527X
pISSN - 0736-0266
DOI - 10.1002/jor.1100010107
Subject(s) - resorption , bone resorption , chemistry , denervation , osteoid , femur , endocrinology , calcium , tibia , medicine , anatomy , surgery , organic chemistry
The major effect of immobilization during growth is a smaller bone mass induced by either an increased bone resorption or a decreased bone formation. Using a method of analyzing radioisotopic loss of [ 3 H]tetracycline and [ 3 H]collagen from bone prelabeled in vivo , we compared the amount of bone resorption due to immobilization with bone resorption induced by growth. One hind limb was denervated in growing male rats, 6 weeks of age, that had been chronically prelabeled with [ 3 H]tetracycline, 45 calcium, and [ 3 H]proline. The total radioactivity of the whole femur and tibia/fibula from the denervated limb was compared with that from bones of the control limb at 0, 1, 2, 4, and 8 weeks after denervation. The effect of growth on bone formation was measured by net increases in bone length, volume, and mass of matrix and mineral. Experimental bones had a significantly smaller volume and mass. Bone resorption was much greater during growth modeling than during denervation. The additional bone resorption induced by denervation was a small fraction (one‐fourth) of the resorption induced by growth. Denervation during growth resulted in less bone being formed due to a smaller gain in matrix and mineral mass as a result of a reduction in bone formation.