Premium
Isolation and screening for alkaline thermostable xylanases
Author(s) -
Subramaniyan S.,
Prema P.,
Ramakrishna S. V.
Publication year - 1997
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620370610
Subject(s) - xylanase , xylan , bran , isolation (microbiology) , agar plate , agar , microorganism , food science , chemistry , enzyme , liquid medium , bacteria , chromatography , biochemistry , microbiology and biotechnology , biology , organic chemistry , raw material , genetics
Screening of xylanase producing microorganisms from soil samples and hemicellulosic materials were carried out in three stages using wheat bran extract agar medium, xylan agar medium and liquid medium with xylan. One of the isolates, identified as Bacillus SSP‐34 produced 100 times more xylanase activity than the other isolates. Maximum enzyme production by this isolate was observed after 102 hours of growth. The optimum pH for the crude xylanase preparation range from 6–8 and optimum temperature was at 50 °C. The enzyme was stable up to 20 minutes at 50 °C and at pH range 4.5–9.0 for more than 2 hours.