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Phenoxyacetic acid induces glutathione‐dependent detoxification and depletes the glutathione pool in Penicillium chrysogenum
Author(s) -
Emri Tamás,
Pócsi István,
Szentirmai Attila
Publication year - 1997
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620370306
Subject(s) - glutathione , penicillium chrysogenum , glutathione reductase , biochemistry , chemistry , detoxification (alternative medicine) , glutathione synthetase , enzyme , iodoacetic acid , glutathione peroxidase , medicine , alternative medicine , pathology
Enzymes of the glutathione‐dependent detoxification pathway (glutathione S‐transferase and γ‐glutamyl‐transpeptidase) 1 ) were induced, and the glutathione pool was completely depleted by phenoxyacetic acid in Penicillium chrysogenum mycelia incubated for 15 h in a culture medium containing lactose as a carbon source and sodium glutamate as a nitrogen source. A significant increase in both the oxidised glutathione concentrations and the glutathione reductase activities were also observed. 1‐Chloro‐2,4‐dinitrobenzene ‐ a potent substrate and inducer of glutathione S‐transferase ‐ initiated very similar physiological changes but no β‐lactam production could be detected in this case. When (NH 4 ) 2 HPO 4 was used as a nitrogen source the penicillin biosynthesis was repressed and the induction of γ‐glutamyltranspeptidase by phenoxyacetic acid was hindered considerably.