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Steroid transformation by mutants of Mycobacterium sp. with altered response to antibiotics
Author(s) -
Barthakur Sharmistha,
Roy Monoj K.,
Bera Saphal K.,
Ghosh Anil Ch.
Publication year - 1996
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620360602
Subject(s) - antibiotics , transformation (genetics) , mutant , microbiology and biotechnology , mycobacterium , biology , bacteria , chemistry , genetics , gene
Mycobacterium sp. NRRLB3683 which is capable to convert β‐sitosterol to 1,4‐androstadiene‐3, 17‐dione (ADD) was treated with methyl methane sulfonate and two strains with altered sensitivity to various antibiotics were obtained. One of the strain was steroid 1(2)‐dehydrogenase negative and the other positive. Efficiency of utilization of sterols followed the order β‐sitosterol > cholesterol > soluble cholesterol. The steroid 1(2)‐dehydrogenase negative strain was capable of producing 17KS (AD) from β‐sitosterol and converting AD to testosterone and ADD to AD suggesting the negative role of 1(2)‐dehydrogenase in sterol side chain cleavage and decrease in hydrogenase activity by mutation. But this enzyme can perform the reverse reaction under aerobic condition.