Premium
Soluble cell‐bound and extracellular cyclodextrin glycosyltransferases of Bacillus macerans show identical enzymological characteristics and antigenicity
Author(s) -
Nógrády Noémi,
Pócsi István,
Katona Éva,
Jeney Viktória,
Boross Péter,
Tözsér József,
Fachet József,
Szentirmai Attila
Publication year - 1996
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620360507
Subject(s) - extracellular , biochemistry , lysis , cell wall , glycosyltransferase , enzyme , chemistry , size exclusion chromatography , cell
Soluble cell‐bound and extracellular cyclodextrin glycosyltransferases of Bacillus macerans were purified to homogeneity using the subsequent steps of adsorption on starch, ion‐exchange chromatography and gel filtration. The elution profiles and the relative molecular masses of the enzymes as well as their N‐terminal sequences were found to be identical. Moreover, mouse monoclonal antibodies raised against the soluble cell‐bound enzyme recognised equally well both the cell‐bound and the extracellular enzyme preparations. Regarding that no signal peptide could be detected in either the cell‐bound or the extracellular cyclodextrin glycosyltransferase the enzyme was probably entrapped between the cell membrane and the cell wall in the early stationary phase and was released during cell lysis without any modification.