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Isozyme polymorphism of cellulases in Aspergillus terreus
Author(s) -
Singh S.,
Brar J. K.,
Sandhu D. K.,
Kaur A.
Publication year - 1996
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620360412
Subject(s) - aspergillus terreus , cellulase , isozyme , electrophoresis , polymorphism (computer science) , enzyme , biology , chemistry , glucanase , biochemistry , allele , gene
Abstract Maximum cellulase production in Aspergillus terreus was obtained at a temperature of 28 °C, pH 4.0 and a substrate concentration of 1% CMC. Variability in cellulase enzyme production and isozyme polymorphism of endo‐β‐1,4‐glucanase and β‐1,4‐glucosidase was studied in 45 natural isolates of A. terreus. Different electrophoretic patterns were evident for endoglucanase. Three zones of activity viz EG 1, EG 11 and EG 111 were observed showing different electrophoretic mobilities. Some of the isolates exhibited the presence of null alleles for EG 1. During development EG 1 and EG 11 were observed throughout while EG 111 appeared on the eighth day. For β‐1,4‐glucosidase two zones of activity viz β‐glu 1 and β‐glu 11 were observed. β‐glu 1 showed variable electrophoretic mobilities. β‐glu 1 appeared throughout during development while β‐glu 11 appeared on the twelfth day.