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Biological and molecular characterisation of potential biocontrol strains of Trichoderma
Author(s) -
Turóczi György,
Fekete Csaba,
Kerényi Zoltán,
Nagy Richárd,
Pomázi Andrea,
Hornok László
Publication year - 1996
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620360113
Subject(s) - intraspecific competition , rapd , chitinase , biology , mycelium , strain (injury) , trichoderma , biological pest control , trichoderma viride , trichoderma harzianum , genetic divergence , microbiology and biotechnology , botany , genetic diversity , genetics , gene , ecology , population , demography , anatomy , sociology
Thirty‐four strains of seven species of Trichoderma isolated from various fungal sources were compared for direct mycoparasitic activity (MPA), chitinase production and antibiotic activity (ANA) in order to choose the most appropriate partners for a strain‐breeding programme. Within species genetic differences were also assesses in T. hamatum, T. harzianum and T. viride by means of random amplification of polymorphic DNA (RAPD). Endochitinase activities of the Trichoderma strains ranged between 20.4 and 1264.5 units/g dry weight of mycelium. The correlation between MPA and chitinase activity was not unambiguous and no correlation existed between MPA and ANA. The RAPD patterns of T. viride strains were highly variable, while isolates of T. harzianum proved to be more uniform; T. hamatum revealed remarkable intraspecific divergence. All these three comprised certain pairs of strains that are promising participants of a strain‐improving programme, since their strong genetic affinities offer good changes for combining their contrasted biocontrol traits.