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A rapid method for differentiation of Xanthomonas campestris pv. mangiferaeindicae from other Xanthomonads and mango phylloplane inhabitants
Author(s) -
Sanders Gina M.,
Korsten L.
Publication year - 1995
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620350509
Subject(s) - phyllosphere , xanthomonas campestris , biology , microbiology and biotechnology , botany , xanthomonas , bacteria , genetics
A rapid method for differentiation of Xanthomonas campestris pv. mangiferaeindicae from other Xanthomonads and phylloplane inhabitants is described. Several selective media were evaluated for selective enrichment of X. campestris pv. mangiferaeindicae from mango plants. Boost broth supplemented with cycloheximide, methyl violet and methyl green enhanced growth of epiphytic X. campestris pv. mangiferaeindicae but not other phylloplane organisms. The identity of X. campestris pv. mangiferaeindicae selectively enriched from mango plant material was confirmed with monoclonal antibodies in an Enzyme‐Linked Immunosorbent Assay. Preliminary field evaluations showed that selective enrichment and confirmation by ELISA could successfully detect latent infections of X. campestris pv. mangiferaeindicae in mango plants.