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Isolation of porcine adenovirus as a candidate of 5th serotype
Author(s) -
Kadoi K.,
Inoue Y.,
Ikeda T.,
Kamata H.,
Yukawa M.,
Iwabuchi M.,
Inaba Y.
Publication year - 1995
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620350314
Subject(s) - virology , biology , serotype , virus , agarose , adenoviridae , serology , mastadenovirus , strain (injury) , agarose gel electrophoresis , isolation (microbiology) , microbiology and biotechnology , dna , antibody , immunology , genetics , anatomy , gene , recombinant dna
A strain of cytopathic virus, named strain TG/K79, was isolated from the brain of a newborn piglet, pure Hampshire breed, which died shortly after birth. The physicochemical property of virus was considered to be that of the family Adenoviridae. A significant difference between our isolate and 4 reference porcine adenoviruses was demonstrated by cross‐seroneutralization test Differences between TG/K79 and other porcine adenoviruses were also seen in electrophoretic patterns of viral DNA in agarose gel after digested by restiction endonucleases. Two SPF pigs, 2‐month‐old, experimentally infected via intranasal showed a fever and a hemorrhagic enteritis. A serological survey indicates that at least swine in the farm where the virus was isolated have been highly contaminated.