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Effect of increased ppGpp concentration on DNA replication of different replicons in Escherichia coli
Author(s) -
Herman Anna,
Wèrzyn Grzegorz
Publication year - 1995
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620350110
Subject(s) - replicon , cole1 , plasmid , stringent response , biology , escherichia coli , dna replication , bacteria , dna , gene , guanosine , microbiology and biotechnology , biochemistry , genetics
The plasmids harbouring the rel A gene under an inducible promoter allowed us to increase the guanosine 5′‐diphosphate‐3′‐diphosphate (ppGpp) concentration in Escherichia coli cells without any starvation and thus, to directly investigate the effect of ppGpp on DNA replication. We studied all types of replicons which were investigated previously in amino acid‐starved bacteria and found that ColE1, ori C, λ plasmid and pSC101 but not RK2 replicons are sensitive to high ppGpp level. To our knowledge, this paper presents the first direct evidence that replication of most, but not all, replicons is dependent on ppGpp concentration and thus, is under stringent control.