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Ecological and physiological studies on thermophilic bacilli from sulfataric hot springs of central Italy
Author(s) -
Canganella Francesco,
Trovatelli Luigi Daniele
Publication year - 1995
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620350105
Subject(s) - thermophile , bacilli , spore , xylose , biology , microorganism , bacillus (shape) , bacteria , microbiology and biotechnology , starch , yeast extract , agar , agar plate , amylase , yeast , clostridium , food science , fermentation , biochemistry , enzyme , genetics
Abstract Hot springs located in a geothermal area of central Italy were investigated with respect to the total number of thermophilic microorganisms as well as to the presence of amylolytic and pullulolytic bacteria. A number of motile thermophilic spore‐forming bacteria were isolated from different sites. The aerobic organisms grew optimally at 60 −65°C on yeast extract‐trypticase and/or xylose‐starch supplemented mineral medium and spores were mostly observed on agar plates after prolonged incubation. According to the results based on phenotypic characterization, G + C% and lipid analyses all the aerobic isolates belong to the genus Bacillus and are characterized by an unusual G + C content ranging between 33.4 ‐ 38.8. A limited number of anaerobic organisms were also isolated and investigated for the presence of amylolytic and pullulolytic activity. According to physiological tests these isolates were identified as Clostridium thermohydrosulfuricum ; when grown on starch, they produced thermostable α‐amylases, pullulanases and α‐glucosidases.