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Mutations affecting gluconate catabolism in Escherichia coli . Genetic mapping of loci for the low affinity transport and the thermoresistant gluconokinase
Author(s) -
De Rekarte Usua D.,
Cortés Marieen,
Porco Antonietta,
Niño Gustavo,
Istúriz Tomás
Publication year - 1994
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620340602
Subject(s) - escherichia coli , catabolism , genetics , biology , mutation , escherichia coli proteins , gene , chemistry , biochemistry , metabolism
The isolation and properties of strains of Escherichia coli carrying mutations affecting either the low affinity transport for gluconate (gene gntU) or the thermoresistant gluconokinase (gene gntK) are described. A lesion of each type was genetically characterized by transduction experiments. Both mutations mapped in the asd region, and the order was malA‐glpD‐asd‐gntU‐gntK, with the last two markers at about min 75.78 and 75.86 on the map, respectively. Mutations altering specifically gntU have not been previously reported.