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Characterization of a Tn 925 ‐induced mutant of Staphylococcus aureus altered in exoprotein production
Author(s) -
Giraudo Ana T.,
Martínez Gabriela L.,
Calzolari Aldo,
Nagel Rosa
Publication year - 1994
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620340507
Subject(s) - mutant , hemolysin , microbiology and biotechnology , staphylococcus aureus , virulence , biology , coagulase , plasmid , transduction (biophysics) , proteases , staphylococcus , enzyme , gene , bacteria , biochemistry , genetics
An insertional mutant was isolated from a bovine Staphylococcus aureus strain after membrane‐mating with a Streptococcus faecalis strain carrying conjugative plasmid pCF10: : Tn 925 . This mutant, designated RC128, showed enhanced production of α‐hemolysin and proteases and decreased production of coagulase, extracellular protein A, DNase, lipase and δ‐hemolysin. No difference was found in the production of β‐hemolysin. Both, Southern blot analysis and transfer of the pleiotropic mutant phenotype by transduction, indicated that the mutation was originated from a single insertion of transposon Tn 925 . The LD50 determined by intraperitoneal administration in mice showed that mutant RC128 was slightly less virulent than its parental strain.