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A method for screening diacetyl and acetoin‐producing bacteria on agar plates
Author(s) -
Phalip V.,
Schmitt P.,
Diviès C.
Publication year - 1994
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620340412
Subject(s) - acetoin , diacetyl , bacteria , agar , chemistry , agar plate , chromatography , biochemistry , biology , fermentation , genetics
A simple method for screening bacteria for diacetyl and acetoin production was developed. This method is based on the ability of diacetyl and acetoin to form a red insoluble complex with α‐naphthol in the presence of creatine. Addition of carboxymethyl‐cellulose containing calcium citrate in the medium allowed discrimination between citrate utilizing and non‐utilizing bacteria.