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A revised map location for the histidine utilization genes in Pseudomonas putida
Author(s) -
King Robin S.,
Sechrist Lynne L.,
Phillips Allen T.
Publication year - 1994
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620340408
Subject(s) - gene , pseudomonas putida , genetics , biology , cloning (programming) , chromosome , histidine , amino acid , computer science , programming language
The histidine utilization genes hutH and hutU of Pseudomonas putida ATCC 12633 have been mapped by interrupted mating and transduction to a location at approximately 43 minutes on the chromosome, closely linked to ser‐800 and met‐400 markers previously shown to be at 46 and 42 minutes, respectively. Since restriction enzyme mapping and cloning results have established that all genes associated with the hut pathway are contiguous, earlier maps of this strain which place these genes near 10 minutes on the chromosome in a superoperonic catabolic cluster are in error.