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Optimization of alkaline amylase production by thermophilic Bacillus stearothermophilus AN 002
Author(s) -
Bezbaruah R. L.,
Gogoi B. K.,
Pillai K. R.
Publication year - 1994
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620340302
Subject(s) - amylase , starch , chemistry , thermophile , corn steep liquor , aeration , food science , industrial fermentation , chromatography , biochemistry , fermentation , enzyme , organic chemistry
Bacillus stearothermophilus AN 002 secreted thermostable alkaline amylase into a liquid growth medium containing 2 g/l starch. Amylase activity was highest at early stationary phase of growth. Among the various carbohydrates tested maximum amylase production was obtained with starch. When starch was replaced by other carbon sources, amylase production was reduced. Peptone and corn steep liquor (CSL) were the ideal nitrogen sources for amylase production by this strain. Of the phosphate sources tested, (NH 4 ) 2 HPO 4 showed best restults. Amylase production was highest in a laboratory fermenter at a initial pH of 6.5 and at a constant pH of 7.0. The optimum aeration and agitation for amylase production were 0.66 v/v/m and 400 rev/min, respectively. Maximum growth was obtained at 55 °C and maximum amylase was produced at 50 °C. The amylase was found to be stable between pH 6.0 to 11.0.