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Pseudomonas acidovorans : a bacterium capable of mineralizing 2‐chloroaniline
Author(s) -
Hinteregger Christel,
Loidl Michael,
Streichsbier Franz
Publication year - 1994
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620340203
Subject(s) - chemistry , catechol , ammonium , strain (injury) , bacteria , mineralization (soil science) , pseudomonas , biodegradation , ammonium chloride , substrate (aquarium) , nuclear chemistry , elution , hydrolysis , pseudomonadaceae , cellulose , chromatography , nitrogen , biochemistry , organic chemistry , biology , ecology , genetics , anatomy
Prolonged adaptation of Ca‐alginate immobilized cells of Pseudomonas acidovorans CA28 to a mixture of 3‐chloroaniline (3‐CA) 1 ) and 2‐CA and subsequently to 2‐CA as sole substrate led to the isolation of another strain, termed CA50 with the additional capability of utilizing 2‐CA as sole source of carbon, nitrogen, and energy. Batch‐degradation of 190 mg/l of 2‐CA at pH 6.1 by this newly isolated strain was achieved within 3 days, at higher concentrations up to 0.6 g/l increasing lag‐phases and degradation periods were observed. Except chloride and ammonium no further metabolites were detectable in the medium. Mineralization of 2‐CA proceeds via the modified ortho ‐cleavage pathway as demonstrated by the presence of catechol 1,2‐dioxygenase (C120) activity, which is characterized by its substrate specificity and elution behaviour on DEAE‐cellulose.