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Investigations of the oxygen supply in Ca‐alginate beads and microcapsules loaded with Penicillium raistrickii using a microelectrode
Author(s) -
Irrgang S.,
Baumgärtl H.,
Schlosser D.,
Zimelka W.,
Schmauder H.P.
Publication year - 1993
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620330506
Subject(s) - microelectrode , oxygen , mycelium , chemistry , hydroxylation , bioreactor , limiting oxygen concentration , glucuronic acid , chromatography , biophysics , chemical engineering , biochemistry , biology , electrode , organic chemistry , botany , enzyme , polysaccharide , engineering
The oxygen supply of free, Ca‐alginate entrapped and microencapsulated mycelia of Penicillium raistrickii i 477 capable of 15α‐hydroxylation of 13‐ethyl‐gon‐4‐en‐3,17‐dione was investigated. Using an oxygen microelectrode distinct gradients of oxygen within the Ca‐alginate beads as well as the microcapsules were detected. Slope and width of the gradients were investigated in dependence on the kind of immobilization, the culture age and the cell density on or in the carrier as well as the different forms of the oxygen supply in the medium. So it could be shown that large parts of immobilizates, approximately 96% of the diameter of both types, were oxygen‐free. In comparison with free mycelia, the lower oxygen supply of the immobilized mycelia led to a metabolic shift to fermentative catabolism.