Premium
In vitro coronatine production by several Pseudomonas syringae pv. glycinea isolates
Author(s) -
Nüske Jörg,
Bublitz Friedemann
Publication year - 1993
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620330406
Subject(s) - coronatine , pseudomonas syringae , pseudomonadaceae , pseudomonas , pseudomonadales , biology , microbiology and biotechnology , bacteria , biochemistry , genetics , arabidopsis , pathogen , gene , mutant
Coronatine production by several Pseudomonas syringae pv. glycinea strains has been studied in batch culture. A reversed‐phase high‐performance liquid chromatographic procedure was developed for rapid quantitation of coronatine in culture fluids. Coronatine and coronafacic acid have been recognized from their mass spectra. Three of the strains investigated showed no detectable amounts of coronatine. Four of the examined high producing wild type strains produced much more coronatine as have been already reported for P. syringae pv. glycinea strains, none of them showed detectable coronafacic acid production. Coronatine production is strictly growth associated. Two strains showed a break down of coronatine already at the beginning of the stationary growth phase.