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Dodecylguanidine monoacetate (dodine) causes severe membrane damage in Pseudomonas syringae above the critical micelle concentration
Author(s) -
Cabral João P. S.
Publication year - 1993
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620330402
Subject(s) - pseudomonas syringae , micelle , efflux , chemistry , pulmonary surfactant , phosphate , critical micelle concentration , cationic polymerization , membrane , chromatography , glycerol , amphiphile , biochemistry , organic chemistry , aqueous solution , copolymer , gene , polymer
The release of K + from Pseudomonas syringae cells treated with dodecylguanidine monoacetate (dodine) was followed with a K + ‐selective glass electrode. Treatment of the cells with 5–15 μmol/1 dodine resulted in low levels of K + release, but higher surfactant concentrations caused extensive and rapid K + efflux. Dodine concentrations that caused high K + release also induced significant leakage of inorganic phosphate. The addition of 5–10 μmol/1 dodine also caused an increase in the rate of oxygen consumption in the presence of glycerol or succinate, but an increase in concentration from 10 to 40 μmol/1 resulted in a concomitant decrease in O 2 consumption. The results from this and previous work suggest that dodine inhibits respiration firstly by causing drainage of coenzymes, and then by a direct interaction with the components of the respiratory chain. Previous work showed that above 25 μmol/1, dodine molecules aggregate to form micelles. The results therefore suggests that, in contrast with other cationic amphiphiles, the micellar form of dodine is more damaging to the cytoplasmic membrane than the free molecules.